a)How will you determine if bacteria have been transformed to carr Show more Screening the vector for insert. a)How will you determine if bacteria have been transformed to carry the plasmid?
a)How will you determine if bacteria have been transformed to carr Show more Screening the vector for insert. a)How will you determine if bacteria have been transformed to carry the plasmid?
b)You use EcoRV PacI and choose another enzyme that cuts only once to digest the plasmid and look for the presence of insert. How many bands will be generated using these enzymes and what size will they be? (dont forget to include the size of your inserts) c)Draw a gel showing the relative position of the bands generated by your restriction digest (make sure to include a marker for comparison) Show less
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