The bacterial plasmid pBR322 contains the gene for ampicillin resistance as well as the gene for tet Show more The bacterial plasmid pBR322 contains the gene for ampicillin resistance as well as the gene for tetracycline resistance.
The bacterial plasmid pBR322 contains the gene for ampicillin resistance as well as the gene for tet Show more The bacterial plasmid pBR322 contains the gene for ampicillin resistance as well as the gene for tetracycline resistance.
The plasmid DNA is cut with the restriction enzyme Pst I which cuts just once in the ampicillin resistance gene. The cut plasmid DNA is ligated with Pst I cut Drosophilia DNA to prepare a collection of plasmids containing Drosophila DNA fragments and the mixture is used to transform E. coli cells. A) Which antibiotic should be added to the medium to select cells that have incorporated a plasmid? B) How would you select those colonies that contains plasmids with inserts of Drosophila DNA? Show less
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