What is primary productivity and how is it calculated? In all cases, we require that your work be typed and that you make computer printouts of spreadsheets, graphics, or statistical output. Worksheet – Ecosystem metabolism

What is primary productivity and how is it calculated? In all cases, we require that your work be typed and that you make computer printouts of spreadsheets, graphics, or statistical output.
Worksheet – Ecosystem metabolism

This worksheet is designed to help you to:

i) Calculate average gross primary production for each treatment.
ii) Statistically compare primary production and respiration between treatments.

Answer/address all of the following questions. In all cases, we require that your work be typed

and that you make computer printouts of spreadsheets, graphics, or statistical output. Your TA

may ask you to email your Excel spreadsheet.

What is primary productivity and how is it calculated? (5pt)
What factors affect rates of primary productivity? (5pt)
What are the two main aims of this lab? (3pt)
State two sets of the null and alternative hypotheses for this lab. (2pt)
Present a table showing all system metabolism data. The spreadsheet should
also include calculated GPP, NPP, and R values (mg O2/L/hour) for each pair of bottles. (10pt)

GPP rates with phytoplankton or with periphyton:
(a) Run an appropriate statistical test comparing GPP between bottles with phytoplankton and those with periphyton, and show your output, highlighting the resulting P

value. (5pt)

(b) State whether the P value derived from the statistical test is greater or lesser than

your α value and giving reason, state whether you will reject or fail to reject your

null hypothesis. (5pt)

(c) Based on the statistical significance of your results, formulate a conclusion to

your initial question. (5pt)

(d) Briefly discuss whether or not your results differed from what you expected to

find. (5pt)

(e) Is there a difference between phytoplankton and periphyton metabolism? (5pt)

GPP rates between the full sun and the shade:
(a) Run an appropriate statistical test comparing GPP between bottles with phytoplankton in the light and in the shade then periphyton in the light and in the shade, and show your output, highlighting the resulting P values. (5pt)

(b) State whether the P values derived from the statistical test are greater or lesser than

your α value and giving reason, state whether you will reject or fail to reject your

null hypothesis. (5pt)

(c) Based on the statistical significance of your results, formulate a conclusion to

your initial question. (5pt)

(d) Briefly discuss whether or not your results differed from what you expected to

find. (5pt)

(e) Does light availability limit metabolism in the pond ecosystem that you studied? (5pt)

Our series of light-dark bottle experiments measuring whole system metabolism neglected several major biotic and abiotic components of the aquatic habitats that we studied. What components did we neglect? (25pt)

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