Which of the following factors has most likely contributed to the atrophy of the patient’s muscle cells?

Which of the following factors has most likely contributed to the atrophy of the patient’s muscle cells?

A nurse practitioner employed in a hospitalist notices that a patient is experiencing muscle atrophy following 2 weeks in traction after a motor vehicle accident. Which of the following factors has most likely contributed to the atrophy of the patient’s muscle cells?
High levels of insulin and IGF-1 in the patient’s blood during immobilization
Denervation of the affected muscles during the time of traction
A reduction of skeletal muscle use secondary to the traction treatment
Reduced oxygen consumption and cellular function that ensures muscle cell survival

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