You want to design a cancer killing virus that specifically detect cancer cells miRNA profile and pr Show more You want to design a cancer killing virus that specifically detect cancer cells miRNA profile and produces a toxin gene call Bax which is express by CMV promoter.
You want to design a cancer killing virus that specifically detect cancer cells miRNA profile and pr Show more You want to design a cancer killing virus that specifically detect cancer cells miRNA profile and produces a toxin gene call Bax which is express by CMV promoter.
You will develop a miRNA classifier similar to the one discussed in class to accomplish you goal. You know that the cancer cells very specifically INHIBIT miRNA-1 and miRNA-2. A}Based on this information alone draw a genetic circuit that can recognize the cancer cells. Draw and label the promoter the Bax gene and miRNA binding site. B}You later realize that miRNA-1 and miRNA-2 is not sufficient to specify cancer cells. You find out that cancer cells also over express specifically miRNA-3 and miRNA-4. Draw a genetic circuit that can sense miRNA-1 miRNA-2 miRNA 3 and miRNA-4 to classify cancer cells. Draw and label the promoter the Bax gene and miRNA binding site and other transcription unit necessary. Show less
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